• Model 340  Spectrophotometer (Sequoia-Turner Corporation, 755 Ravendale Dr., Mountain View, CA94043) By

    Naomi Assadian, Xiaoyun Liu, and Juan Pedro Flores

  • Introduction
                Spectrophotometry is the study and analysis of inorganic and organic substances. This includes organisms such as bacteria in solution. Spectrophotometry within the visible light range depends on how light (electromagnetic radiation) is absorbed and transmitted through a solution with an analyte. An understanding of electromagnetic radiation and Lambert-Beer's Law is needed to know how spectroscopy works. In general, when light from the visible range shines through a solution, specific wavelengths will be absorbed and unabsorbed wavelengths will pass through. Transmitted wavelengths are responsible for the color and compliment the color absorbed by the species in solution. Absorbed light has energy associated with it and causes an electron at ground state to be excited to a higher energy level. A spectrophotometer is an instrument used to determine at what wavelengths the sample absorbs light and the intensity of the absorption. Oftentimes, samples must go through preparation and the chemistry of colorimetry becomes useful.

  • Naomi Assadian Xiaoyun Liu Juan Pedro Flores

    If you have an important question, please contact: Naomi Assadian,